Mar, Sara-Preliminary study of a novel nucleoid associated protein in Streptomyces venezuelae. (Dr. Marie Elliot)

Preliminary study of a novel nucleoid associated protein in Streptomyces venezuelae.

While eukaryotes use histones to organize their genomes,  bacteria rely on nucleoid associated proteins (NAPs) to maintain chromosome integrity and regulate gene expression. Characterization of NAPs contributes to understanding of the molecular mechanisms of bacterium to better produce specific products. Streptomyces bacteria are of interest due their industrially important secondary metabolite production. YlqC,  a protein found in Streptomyces venezuelae,  has been hypothesized to be a NAP due its high abundance in Streptomyces coelicolor, a related species. However,  its function has yet to be confirmed. The goal of this study was to investigate the role of the gene,  sven_5287,  which encodes the protein YlqC in S. venezuelae. Experiments were done to compare the phenotypes and growth of wildtype S. venezuelae and a sven_5287 deletion strain. PCR targeting was used to create a sven_5287 deletion strain. Growth curves for the deletion and wildtype strain were constructed using OD600 measurements and compared. Light microscopy was also used to determine the stage of growth at each time point. The phenotypes of the strains were also compared throughout growth on of solid media. The deletion strain demonstrated reduced growth and delayed growth stage progression in comparison to the wildtype. The final optical density of the deletion strain was significantly less than the wildtype. These results indicate that YlqC has a role in the overall growth of S. venezuelae. Further studies are needed to confirm its role as a NAP and antibiotic production.